Tom R. Gaunt, BSc, PhD

Lecturer in Bioinformatics and Molecular Genetics

Bristol Genetic Epidemiology Laboratories,
School of Social and Community Medicine,
Oakfield House,
Oakfield Grove,
Bristol,
BS8 2BN

 tom.gaunt@bristol.ac.uk

Current work

Cardiovascular disease (CVD) is a major cause of mortality in Great Britain. CVD and its risk factors of hypertension, diabetes and obesity are complex traits caused by multiple genetic and environmental components. A relationship is also observed between early growth and adult disease, suggesting that either foetal nutrition influences adult disease or that some genetic factors influence both early growth and adult disease.

In 2007 I was awarded a British Heart Foundation project grant (PG/07/131/24254) to genotype 50,000 single nucleotide polymorphisms in cardiovascular disease candidate genes (the Illumina/IBC HumanCVD Beadchip) within the British Women's Heart and Health Study. This has led to a range of ongoing national and international collaborative projects on the genetic epidemiology of a range of cardiovascular and related phenotypes, including the UCLEB collaboration between UCL, and the Universities of Edinburgh and Bristol.

In 2010 I was awarded a Medical Research Council Project Grant for developing a systems approach to the classification of genes impacting the cardiovascular phenome. This project builds on my previous BHF funded HumanCVD Beadchip work and data, and integrates with the MRC-funded BCSBmed doctoral training centre.

I have interests in epigenomics, transcriptomics and microbiomics in cohort studies, and am currently involved in the Bioinformatics aspects of the "Accessible Resource for Integrated Epigenomic Studies" (ARIES) based on the ALSPAC cohort. This project aims to build a resource of genome-wide methylation data (using the Illumina Infinium 450k methylation array) on 1000 ALSPAC participants and their mothers at several different time-points.

I also have active research interests in bioinformatics, computational biology and statistical genetics, and have developed programs for analysis of linkage disequilibrium and copy number variation. See http://apps.biocompute.org.uk/ for further details.

Previous work

We have previously published initial analyses of the relationship between genetic variation in the Growth Hormone/Placental Lactogen (GH/PL) gene cluster on chromosome 17 and both cardiovascular and metabolic phenotypes. I was awarded a British Heart Foundation Intermediate Fellowship in 2005 to complete comprehensive haplotype, gene copy number and association analyses of the genes within this region. This study gave an insight into the importance of this region in disease and contribute substantially to future research into therapeutic and diagnostic outcomes.

My previous work includes a comprehensive association analysis of the insulin-like growth factor (IGF2) gene and subsequent work on haplotype analyses of the TH-INS-IGF2 region of chromosome 11. This work demonstrated the importance of this region in the predisposition to weight gain in middle-aged men.

I have also been involved in a project on splicing of the insulin gene (INS) in which we demonstrated that the INS-HphI site, frequently used as a marker for the INS minisatellite, affects splicing of the gene.

Finally, I have been involved in a variety of methods development, including work on microplate array diagonal gel electrophoresis, SNP genotyping and copy number variation methodologies (laboratory and bioinformatic aspects).

Funding

PI Grants

Medical Research Council Project Grant G1000427, 2010-13.
British Heart Foundation Project Grant PG/07/131/24254, 2007-10.
British Heart Foundation Intermediate Fellowship FS/05/065, 2005-8.

Other grants (as Co-I)

Biotechnology and Biological Sciences Research Council (BBSRC) Bioinformatics and Biological Resources Fund grant, 2012-13, PIs Prof George Davey Smith and Dr Caroline Relton.
British Heart Foundation Programme Grant, 2010-15, PI Prof Aroon Hingorani.
Wellcome Trust project grant, 2009-11, PI Prof Debbie Lawlor.

Publications

  1. Johnson T, Gaunt TR, Newhouse SJ, Padmanabhan S, Tomaszewski M, Kumari M, Morris RW, Tzoulaki I, O'Brien ET, Poulter NR, Sever P, Shields DC, Thom S, Wannamethee SG, Whincup PH, Brown MJ, Connell JM, Dobson RJ, Howard PJ, Mein CA, Onipinla A, Shaw-Hawkins S, Zhang Y, Smith GD, Day IN, Lawlor DA, Goodall AH; The Cardiogenics Consortium; Fowkes FG, Abecasis GR, Elliott P, Gateva V; The Global BPgen Consortium; Braund PS, Burton PR, Nelson CP, Tobin MD, van der Harst P, Glorioso N, Neuvrith H, Salvi E, Staessen JA, Stucchi A, Devos N, Jeunemaitre X, Plouin PF, Tichet J, Juhanson P, Org E, Putku M, Sober S, Veldre G, Viigimaa M, Levinsson A, Rosengren A, Thelle DS, Hastie CE, Hedner T, Lee WK, Melander O, Wahlstrand B, Hardy R, Wong A, Cooper JA, Palmen J, Chen L, Stewart AF, Wells GA, Westra HJ, Wolfs MG, Clarke R, Franzosi MG, Goel A, Hamsten A, Lathrop M, Peden JF, Seedorf U, Watkins H, Ouwehand WH, Sambrook J, Stephens J, Casas JP, Drenos F, Holmes MV, Kivimaki M, Shah S, Shah T, Talmud PJ, Whittaker J, Wallace C, Delles C, Laan M, Kuh D, Humphries SE, Nyberg F, Cusi D, Roberts R, Newton-Cheh C, Franke L, Stanton AV, Dominiczak AF, Farrall M, Hingorani AD, Samani NJ, Caulfield MJ, Munroe PB. Blood Pressure Loci Identified with a Gene-Centric Array. Am J Hum Genet. 2011 Dec 9;89(6):688-700. Epub 2011 Nov 17.
  2. Raistrick CA, Alharbi KK, Day IN, Gaunt TR. Analysis of Potential Genomic Confounding in Genetic Association Studies and an Online Genomic Confounding Browser (GCB). Ann Hum Genet. 2011 Nov;75(6):723-31.
  3. Shah S*, Nelson CP*, Gaunt TR* [* equal authorship], van der Harst P, Barnes T, Braund PS, Lawlor DA, Casas JP, Padmanabhan S, Drenos F, Kivimaki M, Talmud PJ, Humphries SE, Whittaker J, Morris RW, Whincup PH, Dominiczak A, Munroe PB, Johnson T, Goodall AH, Cambien F, Diemert P, Hengstenberg C, Ouwehand WH, Felix JF, Glazer NL, Tomaszewski M, Burton PR, Tobin MD, van Veldhuisen DJ, de Boer RA, Navis G, van Gilst WH, Mayosi BM, Thompson JR, Kumari M, Macfarlane PW, Day IN*, Hingorani AD*, Samani NJ*. Four Genetic Loci Influencing Electrocardiographic Indices of Left Ventricular Hypertrophy. Circ Cardiovasc Genet. 2011 Sep 30; [Epub ahead of print]
  4. International Consortium for Blood Pressure Genome-Wide Association Studies; [Author 61/839: Gaunt TR] Genetic variants in novel pathways influence blood pressure and cardiovascular disease risk. Nature. 2011 Sep 11;478(7367):103-9.
  5. Rodriguez S, Cook DG, Gaunt TR, Nightingale CM, Whincup PH, Day IN. Combined analysis of CHRNA5, CHRNA3 and CYP2A6 in relation to adolescent smoking behaviour. J Psychopharmacol. 2011 Jul;25(7):915-23.
  6. Alharbi KK, Hou G, Chen XH, Gaunt TR, Syddall HE, Sayer AA, Dennison EM, Phillips DI, Cooper C, Day IN. Population Mutation Scanning of Human GHR by meltMADGE and Identification of a Paucimorphic Variant. Genet Test Mol Biomarkers. 2011 Jun 20; [Epub ahead of print]
  7. Zabaneh D*, Gaunt TR* [*equal authors], Kumari M, Drenos F, Shah S, Berry D, Power C, Hypponen E, Shah T, Palmen J, Pallas J, Talmud PJ, Casas JP, Sofat R, Lowe G, Rumley A, Morris RW, Whincup PH, Rodriguez S, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Kivimaki M, Whittaker J, Hingorani AD, Day IN*, Humphries SE*. Genetic Variants Associated with von Willebrand Factor Levels in Healthy Men and Women Identified Using the HumanCVD BeadChip. Ann Hum Genet. 2011 Apr 28; [Epub ahead of print]
  8. Talmud PJ, Cooper JA, Gaunt T, Holmes MV, Shah S, Palmen J, Drenos F, Shah T, Kumari M, Kivimaki M, Whittaker J, Lawlor DA, Day IN, Hingorani AD, Casas JP, Humphries SE. Variants of ADRA2A are associated with fasting glucose, blood pressure, body mass index and type 2 diabetes risk: meta-analysis of four prospective studies. Diabetologia. 2011 Apr 1; [Epub ahead of print]
  9. Guthrie PA, Gaunt TR, Abdollahi MR, Rodriguez S, Lawlor DA, Smith GD, Day IN. Amplification ratio control system for copy number variation genotyping. Nucleic Acids Res. 2011 Feb 7; [Epub ahead of print]
  10. De Silva NM, Freathy RM, Palmer TM, Donnelly LA, Luan J, Gaunt T, Langenberg C, Weedon MN, Shields B, Knight BA, Ward KJ, Sandhu MS, Harbord RM, McCarthy MI, Smith GD, Ebrahim S, Hattersley AT, Wareham N, Lawlor DA, Morris AD, Palmer CN, Frayling TM. Mendelian Randomization Studies Do Not Support a Role for Raised Circulating Triglyceride Levels Influencing Type 2 Diabetes, Glucose Levels, or Insulin Resistance. Diabetes. 2011 Jan 31; [Epub ahead of print]
  11. Lanktree MB, Guo Y, Murtaza M, Glessner JT, Bailey SD, Onland-Moret NC, Lettre G, Ongen H, Rajagopalan R, Johnson T, Shen H, Nelson CP, Klopp N, Baumert J, Padmanabhan S, Pankratz N, Pankow JS, Shah S, Taylor K, Barnard J, Peters BJ, M Maloney C, Lobmeyer MT, Stanton A, Zafarmand MH, Romaine SP, Mehta A, van Iperen EP, Gong Y, Price TS, Smith EN, Kim CE, Li YR, Asselbergs FW, Atwood LD, Bailey KM, Bhatt D, Bauer F, Behr ER, Bhangale T, Boer JM, Boehm BO, Bradfield JP, Brown M, Braund PS, Burton PR, Carty C, Chandrupatla HR, Chen W, Connell J, Dalgeorgou C, Boer A, Drenos F, Elbers CC, Fang JC, Fox CS, Frackelton EC, Fuchs B, Furlong CE, Gibson Q, Gieger C, Goel A, Grobbee DE, Hastie C, Howard PJ, Huang GH, Johnson WC, Li Q, Kleber ME, Klein BE, Klein R, Kooperberg C, Ky B, Lacroix A, Lanken P, Lathrop M, Li M, Marshall V, Melander O, Mentch FD, J Meyer N, Monda KL, Montpetit A, Murugesan G, Nakayama K, Nondahl D, Onipinla A, Rafelt S, Newhouse SJ, Otieno FG, Patel SR, Putt ME, Rodriguez S, Safa RN, Sawyer DB, Schreiner PJ, Simpson C, Sivapalaratnam S, Srinivasan SR, Suver C, Swergold G, Sweitzer NK, Thomas KA, Thorand B, Timpson NJ, Tischfield S, Tobin M, Tomaszweski M, Verschuren WM, Wallace C, Winkelmann B, Zhang H, Zheng D, Zhang L, Zmuda JM, Clarke R, Balmforth AJ, Danesh J, Day IN, Schork NJ, de Bakker PI, Delles C, Duggan D, Hingorani AD, Hirschhorn JN, Hofker MH, Humphries SE, Kivimaki M, Lawlor DA, Kottke-Marchant K, Mega JL, Mitchell BD, Morrow DA, Palmen J, Redline S, Shields DC, Shuldiner AR, Sleiman PM, Smith GD, Farrall M, Jamshidi Y, Christiani DC, Casas JP, Hall AS, Doevendans PA, D Christie J, Berenson GS, Murray SS, Illig T, Dorn GW 2nd, Cappola TP, Boerwinkle E, Sever P, Rader DJ, Reilly MP, Caulfield M, Talmud PJ, Topol E, Engert JC, Wang K, Dominiczak A, Hamsten A, Curtis SP, Silverstein RL, Lange LA, Sabatine MS, Trip M, Saleheen D, Peden JF, Cruickshanks KJ, Marz W, O'Connell JR, Klungel OH, Wijmenga C, Maitland-van der Zee AH, Schadt EE, Johnson JA, Jarvik GP, Papanicolaou GJ; Hugh Watkins on behalf of PROCARDIS; Grant SF, Munroe PB, North KE, Samani NJ, Koenig W, Gaunt TR, Anand SS, van der Schouw YT; Meena Kumari on behalf of the Whitehall II Study and the WHII 50K Group; Soranzo N, Fitzgerald GA, Reiner A, Hegele RA, Hakonarson H, Keating BJ. Meta-analysis of Dense Genecentric Association Studies Reveals Common and Uncommon Variants Associated with Height. Am J Hum Genet. 2011 Jan 7;88(1):6-18. Epub 2010 Dec 30.
  12. Alharbi KK, Aldahmesh MA, Gaunt TR, Rassoulian H, Ai Guthrie P, Rodriguez S, Boustred CR, Spanakis E, Day IN. MeltMADGE for mutation scanning of specific genes in population studies. Nat Protoc. 2010;5(11):1800-12. Epub 2010 Oct 21.
  13. Raistrick CA, Day IN, Gaunt TR. Genome-Wide Data-Mining of Candidate Human Splice Translational Efficiency Polymorphisms (STEPs) and an Online Database. PLoS One. 2010 Oct 11;5(10). pii: e13340.
  14. Smart-Halajko MC, Robciuc MR, Cooper JA, Jauhiainen M, Kumari M, Kivimaki M, Khaw KT, Boekholdt SM, Wareham NJ, Gaunt TR, Day IN, Braund PS, Nelson CP, Hall AS, Samani NJ, Humphries SE, Ehnholm C, Talmud PJ. The Relationship Between Plasma Angiopoietinlike Protein 4 Levels, Angiopoietinlike Protein 4 Genotype, and Coronary Heart Disease Risk. Arterioscler Thromb Vasc Biol. 2010 Sep 9; [Epub ahead of print]
  15. Rodriguez S, Eiriksdottir G, Gaunt TR, Harris TB, Launer LJ, Gudnason V, Day IN. IGF2BP1, IGF2BP2 and IGF2BP3 genotype, haplotype and genetic model studies in metabolic syndrome traits and diabetes. Growth Horm IGF Res. 2010 Aug;20(4):310-8.
  16. Gaunt TR, Rodriguez S, Guthrie PA, Day IN. An expectation-maximization program for determining allelic spectrum from CNV data (CoNVEM): insights into population allelic architecture and its mutational history. Hum Mutat. 2010 Apr;31(4):414-20.
  17. Talmud PJ, Drenos F, Shah S, Shah T, Palmen J, Verzilli C, Gaunt TR, Pallas J, Lovering R, Li K, Casas JP, Sofat R, Kumari M, Rodriguez S, Johnson T, Newhouse SJ, Dominiczak A, Samani NJ, Caulfield M, Sever P, Stanton A, Shields DC, Padmanabhan S, Melander O, Hastie C, Delles C, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Munroe PB, Day IN, Kivimaki M, Whittaker J, Humphries SE, Hingorani AD; ASCOT investigators; NORDIL investigators; BRIGHT Consortium. Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Am J Hum Genet. 2009 Nov;85(5):628-42.
  18. Dennison EM, Syddall HE, Jameson KA, Sayer AA, Gaunt TR, Rodriguez S, Day IN, Cooper C, Lips MA; Hertfordshire Cohort Study Group. A study of relationships between single nucleotide polymorphisms from the growth hormone-insulin-like growth factor axis and bone mass: the Hertfordshire cohort study. J Rheumatol. 2009 Jul;36(7):1520-6. Epub 2009 Jun 1.
  19. Rodriguez S, Gaunt TR, Day IN. Hardy-Weinberg equilibrium testing of biological ascertainment for Mendelian randomization studies. Am J Epidemiol. 2009 Feb 15;169(4):505-14. Epub 2009 Jan 6.
  20. Lawlor DA, Harbord RM, Timpson NJ, Lowe GD, Rumley A, Gaunt TR, Baker I, Yarnell JW, Kivimaki M, Kumari M, Norman PE, Jamrozik K, Hankey GJ, Almeida OP, Flicker L, Warrington N, Marmot MG, Ben-Shlomo Y, Palmer LJ, Day IN, Ebrahim S, Smith GD. The association of C-reactive protein and CRP genotype with coronary heart disease: findings from five studies with 4,610 cases amongst 18,637 participants. PLoS One. 2008 Aug 20;3(8):e3011.
  21. Abdollahi MR, Huang S, Rodriguez S, Guthrie PA, Smith GD, Ebrahim S, Lawlor DA, Day IN, Gaunt TR. Homogeneous assay of rs4343, an ACE I/D proxy, and an analysis in the British Women's Heart and Health Study (BWHHS). Dis Markers. 2008;24(1):11-7.
  22. Gaunt TR, Rodriguez S, Day IN. Cubic exact solutions for the estimation of pairwise haplotype frequencies: implications for linkage disequilibrium analyses and a web tool 'CubeX'. BMC Bioinformatics. 2007 Nov 2;8:428.
  23. Publication Types: Comment Letter Rodriguez S, Gaunt TR, Vorechovsky I, Kralovicova J, Wood PJ, Day IN. Diabetes. 2007 Sep;56(9):e16; author reply e17.
  24. Rodriguez S, Gaunt TR, Day IN. Molecular genetics of human growth hormone, insulin-like growth factors and their pathways in common disease. Hum Genet. 2007 Aug;122(1):1-21. Epub 2007 May 30.
  25. Huang S, Chen XH, Payne JR, Pennell DJ, Gohlke P, Smith MJ, Day IN, Montgomery HE, Gaunt TR. Haplotype of growth hormone and angiotensin I-converting enzyme genes, serum angiotensin I-converting enzyme and ventricular growth: pathway inference in pharmacogenetics. Pharmacogenet Genomics. 2007 Apr;17(4):291-4.
  26. Ebrahim S, Lawlor DA, Shlomo YB, Timpson N, Harbord R, Christensen M, Baban J, Kiessling M, Day I, Gaunt T, Davey Smith G. Alcohol dehydrogenase type 1C (ADH1C) variants, alcohol consumption traits, HDL-cholesterol and risk of coronary heart disease in women and men: British Women's Heart and Health Study and Caerphilly cohorts. Atherosclerosis. 2008 Feb;196(2):871-8. Epub 2007 Mar 26.
  27. Lips MA, Syddall HE, Gaunt TR, Rodriguez S, Day IN, Cooper C, Dennison EM; Southampton Genetic Epidemiology Research Group. Interaction between birthweight and polymorphism in the calcium-sensing receptor gene in determination of adult bone mass: the Hertfordshire cohort study. J Rheumatol. 2007 Apr;34(4):769-75. Epub 2007 Feb 15.
  28. Abdollahi MR, Lewis RM, Gaunt TR, Cumming DV, Rodriguez S, Rose-Zerilli M, Collins AR, Syddall HE, Howell WM, Cooper C, Godfrey KM, Cameron IT, Day IN. Quantitated transcript haplotypes (QTH) of AGTR1, reduced abundance of mRNA haplotypes containing 1166C (rs5186:A>C), and relevance to metabolic syndrome traits. Hum Mutat. 2007 Apr;28(4):365-73.
  29. Lawlor DA, Day IN, Gaunt TR, Hinks LJ, Timpson N, Ebrahim S, Davey Smith G. The association of the paraoxonase (PON1) Q192R polymorphism with depression in older women: findings from the British Women's Heart and Health Study. J Epidemiol Community Health. 2007 Jan;61(1):85-7.
  30. Zhang W, Maniatis N, Rodriguez S, Miller GJ, Day IN, Gaunt TR, Collins A, Morton NE. Refined association mapping for a quantitative trait: weight in the H19-IGF2-INS-TH region. Ann Hum Genet. 2006 Nov;70(Pt 6):848-56.
  31. Day IN, Rodriguez S, Kralovicova J, Wood PJ, Vorechovsky I, Gaunt TR. Questioning INS VNTR role in obesity and diabetes: subclasses tag IGF2-INS-TH haplotypes; and -23HphI as a STEP (splicing and translational efficiency polymorphism). Physiol Genomics. 2006 Dec 13;28(1):113. Epub 2006 Aug 1.
  32. Gaunt TR, Rodriguez S, Zapata C, Day IN. MIDAS: software for analysis and visualisation of interallelic disequilibrium between multiallelic markers. BMC Bioinformatics. 2006 Apr 27;7:227.
  33. Lawlor DA, Gaunt TR, Hinks LJ, Davey Smith G, Timpson N, Day IN, Ebrahim S. The association of the PON1 Q192R polymorphism with complications and outcomes of pregnancy: findings from the British Women's Heart and Health cohort study. Paediatr Perinat Epidemiol. 2006 May;20(3):244-50.
  34. Christensen MB, Lawlor DA, Gaunt TR, Howell WM, Davey Smith G, Ebrahim S, Day IN. Genotype of galectin 2 (LGALS2) is associated with insulin-glucose profile in the British Women's Heart and Health Study. Diabetologia. 2006 Apr;49(4):673-7. Epub 2006 Feb 9.
  35. Kralovicova J, Gaunt TR, Rodriguez S, Wood PJ, Day IN, Vorechovsky I. Variants in the human insulin gene that affect pre-mRNA splicing: is -23HphI a functional single nucleotide polymorphism at IDDM2? Diabetes. 2006 Jan;55(1):260-4.
  36. Rodriguez S, Huang S, Chen XH, Gaunt TR, Syddall HE, Gilg JA, Miller GJ, Cooper CC, Cook DG, Whincup PH, Day IN. A study of TH01 and IGF2-INS-TH haplotypes in relation to smoking initiation in three independent surveys. Pharmacogenet Genomics. 2006 Jan;16(1):15-23.
  37. Timpson NJ, Lawlor DA, Harbord RM, Gaunt TR, Day IN, Palmer LJ, Hattersley AT, Ebrahim S, Lowe GD, Rumley A, Davey Smith G. C-reactive protein and its role in metabolic syndrome: mendelian randomisation study. Lancet. 2005 Dec 3;366(9501):1954-9.
  38. Rodriguez S, Gaunt TR, Dennison E, Chen XH, Syddall HE, Phillips DI, Cooper C, Day IN. Replication of IGF2-INS-TH*5 haplotype effect on obesity in older men and study of related phenotypes. Eur J Hum Genet. 2006 Jan;14(1):109-16.
  39. Timpson NJ, Christensen M, Lawlor DA, Gaunt TR, Day IN, Ebrahim S, Davey Smith G. TAS2R38 (phenylthiocarbamide) haplotypes, coronary heart disease traits, and eating behavior in the British Women's Heart and Health Study. Am J Clin Nutr. 2005 May;81(5):1005-11.
  40. Abdollahi MR, Gaunt TR, Syddall HE, Cooper C, Phillips DI, Ye S, Day IN. Angiotensin II type I receptor gene polymorphism: anthropometric and metabolic syndrome traits. J Med Genet. 2005 May;42(5):396-401.
  41. Day IN, Chen XH, Gaunt TR, King TH, Voropanov A, Ye S, Rodriguez S, Syddall HE, Sayer AA, Dennison EM, Tabassum F, Barker DJ, Cooper C, Phillips DI. Late life metabolic syndrome, early growth, and common polymorphism in the growth hormone and placental lactogen gene cluster. J Clin Endocrinol Metab. 2004 Nov;89(11):5569-76.
  42. Lawlor DA, Day IN, Gaunt TR, Hinks LJ, Briggs PJ, Kiessling M, Timpson N, Smith GD, Ebrahim S. The association of the PON1 Q192R polymorphism with coronary heart disease: findings from the British Women's Heart and Health cohort study and a meta-analysis. BMC Genet. 2004 Jun 23;5:17.
  43. Rodriguez S, Gaunt TR, O'Dell SD, Chen XH, Gu D, Hawe E, Miller GJ, Humphries SE, Day IN. Haplotypic analyses of the IGF2-INS-TH gene cluster in relation to cardiovascular risk traits. Hum Mol Genet. 2004 Apr 1;13(7):715-25. Epub 2004 Jan 28.
  44. Beghe B, Barton S, Rorke S, Peng Q, Sayers I, Gaunt T, Keith TP, Clough JB, Holgate ST, Holloway JW. Polymorphisms in the interleukin-4 and interleukin-4 receptor alpha chain genes confer susceptibility to asthma and atopy in a Caucasian population. Clin Exp Allergy. 2003 Aug;33(8):1111-7.
  45. Gaunt TR, Hinks LJ, Rassoulian H, Day IN. Manual 768 or 384 well microplate gel 'dry' electrophoresis for PCR checking and SNP genotyping. Nucleic Acids Res. 2003 May 1;31(9):e48.
  46. O'Dell SD, Syddall HE, Sayer AA, Cooper C, Fall CH, Dennison EM, Phillips DI, Gaunt TR, Briggs PJ, Day IN. Null mutation in human ciliary neurotrophic factor gene confers higher body mass index in males. Eur J Hum Genet. 2002 Nov;10(11):749-52.
  47. Gaunt TR, Cooper JA, Miller GJ, Day IN, O'Dell SD. Positive associations between single nucleotide polymorphisms in the IGF2 gene region and body mass index in adult males. Hum Mol Genet. 2001 Jul 1;10(14):1491-501.
  48. Holloway JW, Lonjou C, Beghe B, Peng Q, Gaunt TR, Gomes I, Hall IP, Dewar JC, Wilkinson J, Thomas NS, Holgate ST, Morton NE. Linkage analysis of the 5q31-33 candidate region for asthma in 240 UK families. Genes Immun. 2001 Feb;2(1):20-4.
  49. O'Dell SD, Gaunt TR, Day IN. SNP genotyping by combination of 192-well MADGE, ARMS and computerized gel image analysis. Biotechniques. 2000 Sep;29(3):500-4, 505-6.
  50. Rosalind H. Ganderton, Sandra D. O'Dell, Tom R. Gaunt, Xiao-he Chen, Lesley J. Hinks, Emmanuel Spanakis, Ian N.M. Day, Microplate-array diagonal-gel electrophoresis (MADGE) systems for high-throughput electrophoresis, Technical Tips Online, Volume 5, Issue 1, January 2000, Pages 12-18, ISSN 1366-2120, DOI: 10.1016/S1366-2120(08)70150-8.
  51. Xiao-He Chen, Sandra D. O'Dell, Lesley J. Hinks, Emmanuel Spanakis, Tom R. Gaunt, Rosalind H. Ganderton, Ian N.M. Day, High-resolution MADGE, Technical Tips Online, Volume 6, Issue 1, January 2001, Pages 15-23, ISSN 1366-2120, DOI: 10.1016/S1366-2120(08)70161-2.
  52. Tom R. Gaunt, Lesley J. Hinks, Xiao-he Chen, Sandra D. O'Dell, Emmanuel Spanakis, Rosalind H. Ganderton, Ian N.M. Day, 384-well MADGE for high-throughput DNA-bank studies, Technical Tips Online, Volume 5, Issue 1, January 2000, Pages 35-45, ISSN 1366-2120, DOI: 10.1016/S1366-2120(08)70155-7.